{"categories":["\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9"],"title":"\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u7d50\u679c\u6bd4\u8f03\u3010BAM\u30bd\u30fc\u30c8\u7de8\u30fb1\u3011","html":"<iframe src=\"https://hatenablog-parts.com/embed?url=https%3A%2F%2Fstaffblog.amelieff.jp%2Fentry%2F2013%2F03%2F27%2F104248\" title=\"\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u7d50\u679c\u6bd4\u8f03\u3010BAM\u30bd\u30fc\u30c8\u7de8\u30fb1\u3011 - \u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u5b9f\u8df5\u30e9\u30dc\uff5c\u30a2\u30e1\u30ea\u30a8\u30d5\u6280\u8853\u30d6\u30ed\u30b0\" class=\"embed-card embed-blogcard\" scrolling=\"no\" frameborder=\"0\" style=\"display: block; width: 100%; height: 190px; max-width: 500px; margin: 10px 0px;\"></iframe>","blog_url":"https://staffblog.amelieff.jp/","url":"https://staffblog.amelieff.jp/entry/2013/03/27/104248","author_name":"amelieff","author_url":"https://blog.hatena.ne.jp/amelieff/","width":"100%","description":"NGS\u89e3\u6790\u3067\u306f\u3001\u540c\u3058\u51e6\u7406\u3092\u884c\u3046\u306e\u306b\u3044\u304f\u3064\u3082\u306e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u304c\u3042\u3063\u3066\u3001\u3069\u308c\u3092\u4f7f\u3063\u305f\u3089\u3044\u3044\u306e\u304b\u8ff7\u3046\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002 \u4f8b\u3048\u3070BAM\u30d5\u30a1\u30a4\u30eb\u3092\u30b2\u30ce\u30e0\u30dd\u30b8\u30b7\u30e7\u30f3\u3067\u30bd\u30fc\u30c8\u3059\u308b\u5834\u5408\u3001\u4ee5\u4e0b\u306e\u9078\u629e\u80a2\u304c\u3042\u308a\u307e\u3059\u3002 1. samtools sort\u3092\u4f7f\u3046 2. picard tools\u306eSortSam.jar\u3092\u4f7f\u3046 \u540c\u3058BAM\u30d5\u30a1\u30a4\u30eb\u3092\u7570\u306a\u308b\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3067\u30bd\u30fc\u30c8\u3057\u3066\u3082\u3001\u7d50\u679c\u306f\u540c\u3058\u306b\u306a\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f \u30c6\u30b9\u30c8\u30c7\u30fc\u30bf\u3068\u3057\u3066\u3001NCBI SRA\u306eSRR077861\u306e\u5148\u982d1000\u30ea\u30fc\u30c9\u3092hg19\u306bBWA\u3067\u30de\u30c3\u30d4\u30f3\u30b0\u3057\u305f\u7d50\u679c\u306eBAM\u3092\u4f7f\u3044\u307e\u3057\u305f\u3002 \u30fb\u30c6\u30b9\u30c8\u30c7\u30fc\u30bf sample.bam\uff08222,146 bytes\uff09 \u3010samtoo\u2026","image_url":null,"blog_title":"\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u5b9f\u8df5\u30e9\u30dc\uff5c\u30a2\u30e1\u30ea\u30a8\u30d5\u6280\u8853\u30d6\u30ed\u30b0","provider_url":"https://hatena.blog","height":"190","version":"1.0","published":"2013-03-27 10:42:48","provider_name":"Hatena Blog","type":"rich"}